![]() ![]() Sequential supplementation with 100 ng/ml IL6 at both day 1 and 5 (for a total of 200 ng/ml IL6) increased ( P < 0.05) ICM cell number to a greater extent than supplementing IL6 at a single time period in one study but not a second study. No increase in ICM or TE cell number was observed after supplementation of 1 or 10 ng/ml IL6 beginning at either day 1 or 5. Supplementing 100 ng/ml recombinant bovine IL6 to in vitro-produced bovine embryos at day 1, 3 or 5 increased ( P < 0.05) inner cell mass (ICM) cell number and the ICM:trophectoderm (TE) ratio but not TE cell number. Transcripts for IL6 and its two cognate receptor subunits ( IL6R, IL6ST) were confirmed in bovine embryos from the 1-cell to blastocyst stages. This work was undertaken to elucidate the role of IL6 during in vitro bovine embryo production. It was unclear whether IL6 serves as an embryokine in cultured bovine embryos. Work in other species suggests that interleukin-6 (IL6) promotes early embryo development. ![]()
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March 2023
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